The field of gene therapy has experienced an insurgence of attention for its widespread ability to regulate gene expression by targeting genomic DNA, messenger RNA, microRNA, and short-interfering RNA for treating malignant and non-malignant disorders.Numerous nucleic acid analogs have been developed to target coding USB or non-coding sequences of the human genome for gene regulation.However, broader clinical applications of nucleic acid analogs have been limited due to their poor cell Lecithin or organ-specific delivery.To resolve these issues, non-viral vectors based on nanoparticles, liposomes, and polyplexes have been developed to date.This review is centered on non-viral vectors mainly comprising of cationic lipids and polymers for nucleic acid-based delivery for numerous gene therapy-based applications.